Evaluation of morphokinetic characteristics of zona pellucida free mouse pre-implantation embryos using time-lapse monitoring system
نویسندگان
چکیده
منابع مشابه
Development of early mouse and rabbit embryos without zona pellucida.
Criteria for assessing embryo vitality of denuded rabbit and mouse embryos in vitro were: volume of the embryo, number of cells and number of metaphase plates after 24 and 48 h in culture. Mouse embryos grew normally, with and without the zona pellucida, but denuded rabbit embryos showed reduced growth (volume and cell number) and failure to implant in synchronized recipients.
متن کاملRevealing the secret life of pre-implantation embryos by time-lapse monitoring: A review
High implantation success following in vitro fertilization cycles are achieved via the transfer of embryos with the highest developmental competence. Multiple pregnancies as a result of the transfer of several embryos per cycle accompany with various complication. Thus, single-embryo transfer (SET) is the preferred practice in assisted reproductive technique (ART) treatment. In order to improve...
متن کاملMelatonin upregulates ErbB1 and ErbB4, two primary implantation receptors, in pre-implantation mouse embryos
Objective(s): To evaluated the effects of melatonin on early embryo competence and the expression rate of the primary implantation receptors (ErbB1 and ErbB4). Materials and Methods: Two-cell mouse embryos were cultured in 3 groups: simple media, melatonin-treated (10-9 M melatonin) and Luzindole-treated (10-9 M luzindole). Then, the rate of ErbB1 and ErbB4 gene and protein expression, the leve...
متن کاملTime-lapse monitoring reveals that vitrification increases the frequency of contraction during the pre-hatching stage in mouse embryos
Contraction during the blastocyst stage is observed during embryonic development of various mammals, including humans, but the physiological role of this process is not well understood. Using time-lapse monitoring (TLM), we studied the influence of vitrification and contractions on embryonic development in mice. Mouse embryos were cultured at the 2-cell stage. At the 8-cell stage, embryos were ...
متن کاملComparison of the effect of Growth Factors on the Development of Pre-implantation Embryos of Vitrified Mouse
Purpose: To improve the development of frozen mouse embryos by adding fibroblast growth factors (FGF) and hepatocytes (HGF) to the culture medium. Materials and Methods: Two-cell mouse embryos obtained from the fallopian tube and frozen by cryotopic method were treated in T6 medium with a dose of 20 ng / ml FGF and 20 ng / ml HGF. The results of their culture were compared with control groups a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The International Journal of Developmental Biology
سال: 2020
ISSN: 0214-6282
DOI: 10.1387/ijdb.190188jj